MyGlo™ Reagent Reader
Simplify Setup and Analysis of Cell Health Assays with a Personal 96-Well Device Powered by the ProNect™ Data Platform
- Sensitive reagent reader and intuitive apps are tailored for use with our luminescence assays
- Design, control and analyze experiments remotely—no data transfers required
- Free 10ml sample of CellTiter-Glo® 2.0 Reagent included with your purchase
- Extend product coverage with a 1-year service agreement
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Streamline Your Assays to Get Results Faster
The MyGlo™ Reagent Reader provides an all-in-one solution to streamline your setup and interpretation of CellTiter-Glo® and other luminescent cell health assays.
Cloud-based, assay-specific apps walk you through every step of your assay on MyGlo, including automated data analysis and visualization.
In-App Data Analyses Offer Effortless Insights
MyGlo runs with the ProNect™ Data Platform. Together, they provide a cloud-connected workflow that integrates instrument, assay and data analysis.
Avoid tedious, error-prone manual data analysis and get the insights you need quickly in various formats. For example, when you carry out CellTiter-Glo® Assays with your MyGlo™ Reagent Reader, these data visualizations are automatically generated:
| • Cell viability percentage • Dose response viability curve |
• Isolate single compound • Dose response inhibition curves |
Free Sample Included
For a limited time, receive a free 10ml sample of CellTiter-Glo® 2.0 Cell Viability Assay when you purchase a MyGlo™ Reagent Reader.
Carry Out Your Experiments Whenever You Want
Your MyGlo is ready when you are.
One Time Setup
Create a ProNect™ Data Platform account, install the MyGlo™ setup software on your computer to connect your MyGlo to Wi-Fi and follow the steps in Connect2ProNect for seamless configuration. No waiting for vendor installation, calibration or training.
Compact Footprint
MyGlo is a personal device, the first of its kind for biology laboratories. Its compact footprint—no bigger than a pipette tip box—means you can keep your MyGlo™ Reagent Reader on your bench. No need to share and schedule time on a group instrument.
Analyze Results from Anywhere
The MyGlo™ Reagent Reader's cloud-based user interface gives you the freedom to design experiments, view finished analyses and monitor assay progress from any desktop computer with an internet connection.
Your Go-To Assays Are Now Even Easier
MyGlo™ Reagent Reader and its apps are tailored specifically for select Promega assays and protocols.
CellTiter-Glo® App
The CellTiter-Glo® App is compatible with all versions of our CellTiter-Glo® Cell Viability Assay.
- The original CellTiter-Glo® Assay
- The highly stable CellTiter-Glo® 2.0 Assay
- The CellTiter-Glo® Assay optimized for 3D cell cultures
CellTiter-Glo® Assays
CellTiter-Glo® Assays determine the number of viable cells in culture using bioluminescent technology to quantitate the amount of ATP, an indicator of metabolically active cells.
- Cited in over 30,000 publications over 20+ years
- Simple, 10-minute, add-mix-measure protocol
- Sensitive down to 10 cells per well
Caspase-Glo® 3/7 App
Measure caspase activity with the Caspase-Glo® 3/7 Application—now available on your MyGlo™ Instrument.
The Caspase-Glo® 3/7 App is compatible with the Caspase-Glo® 3/7 Assays.
- The original Caspase-Glo® 3/7 Assay
- The Caspase-Glo® 3/7 3D Assay optimized for 3D cell cultures
Caspase-Glo® 3/7 Assays
The Caspase-Glo® 3/7 Assays detect caspase-3/7 activity of cells in culture to quantitate the amount of luminescence, which is proportional to the amount of caspase activity present, an indicator of apoptosis.
- High-sensitivity caspase assays
- Simple add-mix-measure protocols
- Easily scalable formats
App Specifications and Performance
CellTiter-Glo® Assay
Microplate Types: 96-well, white | Readout Orientation: top | Sensitivity: 10 cells/well
Performance Data
Jurkat cells were seeded in all-white 96-well plates from 10 cells/well to 100,000 cells/well (n=8 replicates). Cell viability was determined by adding an equal volume of CellTiter-Glo® or CellTiter-Glo® 2.0 Reagent to all wells, and the signal was read on a MyGlo™ Reagent Reader. These graphs were generated using Microsoft Excel.
Jurkat cells were seeded in all-white 96-well plates at 10,000 cells/well and treated with Bortezomib, Palbociclib or Anti-Fas (human, activating) clone CH11 for 24 hours. Data were fit using four-parameter logistic curves. Data were generated using the MyGlo™ Reagent Reader with the ProNect™ Data Platform.
Caspase-Glo® 3/7 Assay
Microplate Types: 96-well, white | Readout Orientation: top | Sensitivity: ~30 apoptotic cells
Performance Data
Jurkat cells were seeded in an all-white 96-well plate from 10 cells/well to 100,000 cells/well and treated with vehicle or 50ng/ml anti-Fas (clone CH11; EMD Millipore Cat.# 05-201) to induce apoptosis (n = 4 replicates per condition). Caspase-3/7 activity was determined by adding an equal volume of Caspase-Glo® 3/7 Reagent to all wells. Assay signal was read on a MyGlo™ Reagent Reader (integration time = 1 second). These graphs were generated using Microsoft Excel.
Jurkat cells were seeded in an all-white 96-well plate at 10,000 cells/well and treated with increasing concentrations of anti-Fas (clone CH11) for 4.5 hours to induce apoptosis. Subsequently, Caspase-Glo® 3/7 Reagent was added, and data was generated using the MyGlo™ Reagent Reader with the ProNect™ Data Platform. Data were fit using four-parameter logistic regression.
MyGlo isn’t just for measuring cell viability and apoptosis.
More assays and apps are on their way. Your MyGlo™ Reagent Reader will be automatically compatible with new apps—no additional purchase required.
Software and Support
| ProNect™ Data Platform Apps |
|---|
| Find the apps and resources we've built for your ProNect™ Data Platform account. |
| One Call Supports It All |
|---|
| We supply both the reagents and the instrument, so one call to Promega answers any questions you may have. |
If you need additional coverage during or after the MyGlo™ Reagent Reader standard warranty period is over, a MyGlo™ Reagent Reader Service Agreement is available. The MyGlo™ Reagent Reader Service Agreement covers all parts, labor and shipping to and from our authorized service locations. This Agreement does not include repair or replacement necessitated by accident, neglect, misuse, unauthorized repair or modification of the instrument.
If a problem arises with the instrument, please contact Promega Technical Service for support. E-mail: techserv@promega.com. If further action is required, you will be given repair options and assigned a return authorization number. Promega is not responsible for instrumentation returned without an authorization number.
To obtain service during the covered period, please take the following steps:
- Contact Promega Technical Services.
- Carry out tests as suggested by your Technical Services contact.
If it is determined that further action is required, you will be given options and assigned a return authorization number. Promega is not responsible for instrumentation returned without an authorization number.
Before returning the instrument, you will be responsible for cleaning it. If the instrument has been exposed to any chemical hazards, consult the Technical Manual for cleaning instructions.
*May not be available in all countries. Contact your local Promega representative for more information.
Protocols
Complete Protocol
Instrument Specifications
| Requirements | |
|---|---|
| Wi-Fi Connectivity |
MyGlo™ Reagent Reader requires connection to the internet via a Wi-Fi network. Wi-Fi, WPA2 (i.e., WPA2-PSK or WPA2-Personal), 2.4GHz only. 5GHz, 6GHz, WPA3-Personal networks not supported at this time (see MyGlo™ Setup Instructions). |
| ProNect™ Data Platform Account | MyGlo™ Reagent Reader is controlled using apps in the ProNect™ Data Platform. |
| Setup and User Interface |
Connect2ProNect: Desktop application for device setup and management runs on Microsoft® Windows 10 or 11. MacOS® and Virtual Machines not supported. ProNect™ Data Platform and Applications: Works with common desktop internet browsers, including Microsoft® Edge® and Google Chrome®. Mobile and tablet browser experiences not supported at this time. |
| System | |
| Warranty | 12 months; see Operating Manual for details. |
| Regulatory Compliance | For Research Use Only. Not for use in diagnostic procedures. |
| Weight | 570g (1.25lb) |
| Dimensions |
98mm × 140mm × 42mm (W × L × H) |
| Power Supply |
5V |
| Power Input | 2.5W |
| Environmental | |
| Shipping Temperature | +4°C to +50°C |
| Operating Temperature | +15°C to +40°C |
| Operating Relative Humidity | ≤80% noncondensing |
| Operating Altitude | <2,000m |
| Performance | |
| Linear Dynamic Range | 6 logs |
| Cross Talk | Average <0.02% |
Other Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
MyGlo™ Reagent Reader
|
MG1000 | 1 × 1 each | |
CellTiter-Glo® 2.0 Reagent |
G9241 | 1 × 10ml | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.SDS
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Resources
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Software and Firmware
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Related Products
Frequently Used With
CellTiter-Glo® 2.0 Cell Viability Assay
Updated CellTiter-Glo® Cell Viability Assay with improved reagent stability. Quantifies cell proliferation based on ATP detection.
G9241, G9242, G9243, MG1010
CellTiter-Glo® One Solution Assay
Gold standard method for determining cell viability based on quantitation of ATP.
G8461, G8462
CellTiter-Glo® Luminescent Cell Viability Assay
Determine the number of viable cells in culture based on quantitation of ATP present.
G7570, G7571, G7572, G7573, MG1010
CellTiter-Glo® 3D Cell Viability Assay
A homogeneous method optimized to assess viability in 3D cell culture.
G9681, G9682, G9683
