Lumit® Active IL-18 (Human) Immunoassay Technical Manual
Instructions for Use of Product(s)
W1910, W1911, W1912
Literature # TM765
The Lumit® Active IL-18 immunoassay is a homogeneous, bioluminescent assay for monitoring released active interleukin 18 (IL-18) directly from human cultured cells without the need for sample transfers or wash steps. IL-18 is highly regulated by an inactivating binding protein, IL-18BP, with very high affinity for active IL-18. To provide an assay selective for active IL-18, IL-18BP is used for detection in this Lumit® immunoassay because it primarily binds to active IL-18. Lumit® reagents can be dispensed directly into microplate wells containing cells and culture medium. Alternatively, medium from cell wells can be transferred to a separate plate for analysis. Assay performance with sample types other than cell culture medium must be determined by the user.
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