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Selenomethionine Protein Labeling Using the Escherichia coli Strain KRX

Kate Qin Zhao, Jim Hartnett and Michael R. Slater
Promega Corporation
Publication Date: 2007

Abstract

With Monster Green® fluorescent protein as a model, we can achieve an average of 90% or greater selenomethionine (Se-Met) incorporation when the GFP is expressed in the E. coli strain KRX. Therefore, it is feasible to produce Se-Met-labeled protein samples for structural biology applications, such as X-ray crystallography, using the Single Step (KRX) Competent Cells.

Promega Notes 96, 24–26.

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